Thin film polycrystalline silicon nanowire biosensors.

نویسندگان

  • Mohammad M A Hakim
  • Marta Lombardini
  • Kai Sun
  • Francesco Giustiniano
  • Peter L Roach
  • Donna E Davies
  • Peter H Howarth
  • Maurits R R de Planque
  • Hywel Morgan
  • Peter Ashburn
چکیده

Polysilicon nanowire biosensors have been fabricated using a top-down process and were used to determine the binding constant of two inflammatory biomarkers. A very low cost nanofabrication process was developed, based on simple and mature photolithography, thin film technology, and plasma etching, enabling an easy route to mass manufacture. Antibody-functionalized nanowire sensors were used to detect the proteins interleukin-8 (IL-8) and tumor necrosis factor-alpha (TNF-α) over a wide range of concentrations, demonstrating excellent sensitivity and selectivity, exemplified by a detection sensitivity of 10 fM in the presence of a 100,000-fold excess of a nontarget protein. Nanowire titration curves gave antibody-antigen dissociation constants in good agreement with low-salt enzyme-linked immunosorbent assays (ELISAs). This fabrication process produces high-quality nanowires that are suitable for low-cost mass production, providing a realistic route to the realization of disposable nanoelectronic point-of-care (PoC) devices.

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عنوان ژورنال:
  • Nano letters

دوره 12 4  شماره 

صفحات  -

تاریخ انتشار 2012